Title: Effects of Jiawei Xiaoqinglong Decoction on expressions of lung TLR4 mRNA in the rats with acute lung injury caused by lipopolysaccharide
Abstract: Objective: To investigate the effects of Jiawei Xiaoqinglong Decoction on lung of expressions of TLR4mRNA in the rats with acute lung injury caused by lipopolysaccharide(LPS).Methods: Seventy-two male Wistar rats were randomly divided into six groups including normal control,LPS model,dexamethasone and Jiawei Xiaoqinglong Decoction(Small,medium and large doses) groups.Each group had two subgroups,4h and 8h after LPS were injected.Each subgroup had six rats.The ALI rat model was established by LPS through intravenous tail injection(10mg/kg).The rats in dexamethasone group or Jiawei Xiaoqinglong Decoction group were pretreated by dexamethasone or Jiawei Xiaoqinglong Decoction(Small,medium and large doses) respectively for 3 days before LPS-induced ALI.The TLR4 mRNA was measured by real-time fluorescent Polymerase chain reaction(PCR) while the histopathology of the lung injury was observed by light microscope.Results: Compared with model group,the expression of TLR4 mRNA were obviously decreased in Jiawei Xiaoqinglong Decoction(small and large doses) groups at 8 hour(P0.01).Light microscope observation indicates that there were large areas of pulmonary hemorrhage and necrosis in model group,while in the Jiawei Xiaoqinglong Decoction group,the pathological manifestations were much more ameliorated than those of the model group.The lung bronchiole may inflammte sometimes,and the edema is lightly.PMN and RBC leak little.Conclusion: Jiawei Xiaoqinglong Decoction lessens the injury of lung tissue and has protective effects on rats with ALI induced by LPS,the mechanism is possibly related to the inhibition of the expressions of TLR4 mRNA in injured lung tissues.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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