Title: Study on tissue culture and rapid propagation of Begonia mannii
Abstract: Tissues cultures were carried out using the blade of Begonia mannii in pot as explants.Results showed that explants of blade's which their surfaces treated by 0.1% HgCl_2 for 10 min could obtain low rate of dead and infection,and they could be induced to regenerate plant when they were inoculated in culture medium of 3/4 MS+6-BA 2.5 mg/L+NAA 1.0 mg/L+Agar 7.8 g/L+Sucrose 25 g/L.Theregeneratedplant of B.mannii cultured in 3/4 MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+ Agar 7.8g/L+Sucrose 25g/L had the propagationcoefficientof6~8.An 2.0~3.0cm individual plant with 6~9 strong roots was obtained after the regenerated plants were divided and cultured in 1/2 MS+KT 2.0 mg/L+NAA 0.3 mg/L+Agar 7.8 g/L+Sucrose 25 g/L for 25 days.The optium transplantation medium is Peat+Silt+Sawdust(1∶1∶1)in which the survival rate of transplant test-tube seedling is 95%.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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