Title: Tissue Culture and Rapid Propagation of Mokara
Abstract: The axillary buds of Mokara was used as explants to establish a tissue culture and regeneration system.The results indicated that axillary buds is suitable to sterilized twice mode with 0.1% HgCl2.6-BA 4.0 mg/L+NAA 0.4 mg/L was good for inducing the protocorm-like bodies.Multiplication medium of 6-BA 1.0 mg/L+NAA 0.1 mg/L was the best.6-BA 0.5 mg/L+NAA 0.05 mg/L was the optimum concentration for adventitious bud forming.It was on rooting medium(VW+NAA 0.5 mg/L+coconut water 100 ml/L+banana 40 g/L+potato 30 g/L+sucrose 20 g/L+carrageenan 8.0 g/L+AC 1.5 g/L) that the rooting rate found was 100%.The transplantation surviving rate could reach 95%.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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