Title: Expression and clinical significance of PTEN and VEGF proteins in human gastric carcinoma detected using tissue microarray
Abstract: Objective To investigate the expressions and clinical significance of PTEN and vascular endothelial growth factor (VEGF) proteins in human gastric carcinoma and normal gastric mucosa. Methods Tissue microarray was used in 72 cases of gastric carcinoma and 25 cases of normal gastric mucosa. The expressions of PTEN and VEGF proteins were detected using immunohistochemical S-P method. Results In gastric carcinoma, the positive expression rates of PTEN and VEGF proteins were 45.8%(33/72) and 75%(54/72), while 100%(25/25) and 12%(3/25) in normal mucosa, respectively, showing the expressions of PTEN and VEGF proteins in cancer tissues were significant difference when compared with those in normal mucosa (P 0.01, P 0.01). The expressions of PTEN and VEGF proteins in the well-differentiated and mid-differentiated carcinoma groups were 68.8% and 62.5%; those in poorly differentiated and undifferentiated carcinoma groups were 27.5% and 85.0%, respectively. The expressions of PTEN and VEGF proteins were significantly correlated with infiltrating depth, cell differentiation, lymph node metastasis and clinical stages, but not with gender, ages, tumor volume and histological classification. The expression of PTEN was negatively related to the expression of VEGF in gastric carcinoma (P 0.01). Conclusion PTEN and VEGF proteins are significantly correlated with the clinicopathological characteristics and biologic behaviors in gastric carcinoma. Combined detection of PTEN and VEGF might be helpful to evaluate malignant degree and prognosis of gastric carcinoma. It is believed that tissue microarray is a rapid, economic and accurate tool in screening the clinical tissue specimens on a large scale.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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