Title: Determination of the content of Berberine Hydrochloride by HPLC in Zuojin Capsule
Abstract: Objective To establish a HPLC method for the determination of Berberine Hydrochloride in Zuojin Capsule. Methods The content was determined by HPLC. The ZORBAX 80A Extend-C18 column( 4. 6 mm × 150 mm,5 μm) was used. The mobile phase was 0. 05 mol/L potassium dihydrogen phosphate and acetonitrile( 75: 25). The flow rate was 0. 6 mL / min. The detection wavelength was 350 nm. Results Linearity of Berberine Hydrochloride was found in the range from 0. 2 118 mg to 1. 0 590 mg( r = 0. 999,n = 5). The average recovery rate was 100. 02% and RSD was 0. 19%( n = 5). The minimum content of Berberine Hydrochloride in every Zuojin Capsule was 22. 7 mg. Conclusion The method is simple,fast and stable. It has been proved to be a good method for the determination of Berberine Hydrochloride in Zuojin Capsule.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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