Title: Study on the Colonizational virulence of coccoid Helicobacter Pylori induced by antibiotics
Abstract: Objective To show the virulence related to the
colonization of antibioticinduced coccoid Helicobacter pylori mutant. Methods Five strains of
coccoid H. pylori, which were transformed from bacillary by exposure to antibiotics in
subinhibitory concentrations, were examined by their activities of urease and their adherance to
Hep2 cell, the gene variety of urease A, urease B and hpaA were analysed by PCR. Results
Both activity of urease and adherance to Hep2 cell of coccoid H. pylori were decreased, and the
invasion of coccoid H.pylori into hep2 cell could be seen under etectromicroscope, moreover,
411bp Ure A, 115bp UreB and 375bp hpaA in coccoid H.pylori were all shown to be positive as
by PCR. Conclusion Virulence related to colonization in antibiotic induced coccoid H.pylori
decreases, yet there is no deletion in virulencerelated gene fragments.
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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