Title: Effect of ischemic postconditioning on apoptosis after focal cerebral ischemia reperfusion in rat
Abstract: Objective:To investigate the effect of ischemic postconditioning on apoptosis after focal cerebral ischemia reperfusion in rat.Methods:A rat model of focal cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion using modified filament method.Male Sprague-Dawley rats were randomized into 3 groups(n=10):sham-operate(sham) group,ischemia/reperfusion(I/R) group and ischemic postconditioning(IP) group.TUNEL assay was employed to examine the cell apoptosis and Western blotting analysis were used to detect the expression of P38 and Caspase-3 proteins.Results:The apoptotic cells and protein expression of P38 and Caspase-3 increased markedly after cerebral ischemia-reperfusion injury in rats.While the apoptotic cells and protein expression of P38 and Caspase-3 decreased significantly in the IP group compared with the I/R group(P0.01).Conclusion:Ischemic postconditioning decrease the protein expression of P38 and Caspase-3 after focal cerebral ischemia reperfusion in rat,which might be an important mechanism of apoptosis.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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