Title: Rhizosphere Colonization and Effects of an Endophytic Fungus DZJ07 From Eucommia ulmoides on Enzyme Activities of Wheat Root
Abstract: The colonization of antagonistic endophytes in the plants rhizosphere was a critical factor to prevent plant diseases biologically. In the study,the endophytic fungi DZJ07 showing strong activity against wheat Rhizoctonia cerealis was marked with carbendazim by the concentration gradient method,the colonization property of biocontrol strain DZJ07 in wheat rhizosphere was analysed,and the effect of colonization of mutant DZJ07-2 in the wheat rhizosphere on related enzyme activity of wheat roots in different growth stages was researched through pot experiments. The results indicated the mutant DZJ07-2 could tolerate 80% carbendazim at concentration of 200 μg · m L-1and had the stabilities of heredity,the mutant strain successfully colonized in the wheat rhizosphere,and the population reached to the highest level after 20 days,with 6. 33 × 102cfu·g-1in the soil. Then the activities of PPO,POD and PAL of wheat roots treated with of DZJ07-2 were significantly higher than that of normal control groups,and colonization of DZJ07-2 in the wheat rhizosphere could reduce significantly the incidence of wheat sharp eyespot,the incidence of wheat sharp eyespot by DZJ07-2 was 13. 36%,which was lower than that of the normal control groups. The results afforded scientific basis for its application and the further research on bio-controlling mechanism.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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