Title: Studies on Quality Standard of Radix Serratulae Chinensis
Abstract:Objective To establish the quality standard of Radix Serratulae Chinensis.Methods The micromethod and TLC were used for qualitative identification.Meanwhile,a RP-HPLC analysis was applied for quantita...Objective To establish the quality standard of Radix Serratulae Chinensis.Methods The micromethod and TLC were used for qualitative identification.Meanwhile,a RP-HPLC analysis was applied for quantitative determination of ecdysterone.The analysis was carried on Sepax Sapphire-C18 column(250 mm×4.60 mm,5 μm),with mobile phase consisted of methanol-water solution(45:55) at a flow rate of 1.0 mL/min and the detective wavelength was 248 nm,the column temperature is 30℃.Results Kinds of inulins obviously distributed in Radix Serratulae Chinensis,the characteristic of identification by TLC was distinct and highly specific.The linear range of ecdysterone was 0.1904-2.0940 μg(r=0.9999),the average recovery was 99.73%,RSD was 2.57%(n=6),and repetition RSD was 0.64%(n=6).Conclusion RP-HPLC analysis can be applied for determination of ecdysterone,and the method is simple,accurate and reproducible,and suitable for the quality control of Radix Serratulae Chinensis.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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Cited By Count: 1
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