Title: HPLC determination of four active components in Huoxue Zhitong Capsules
Abstract: AIM To establish a method for determing notoginsenoside R1、ginsenoside Rg1、ginsenoside Rb1、 ferulic acid in Huoxue Zhitong Capsules(Angelicae sinensis Radix,Notoginseng Radix et Rhizoma,Olibarum,Eupolyphaga or steleophaga,Borneolum Syntheticm,Pyritum).METHODS The HPLC was carried out on kromasilTM C18 column with mobile phase of acetonitrile-water(0-12 min,19∶ 81;12-60 min,36∶ 64)for notoginsenoside R1,ginsenoside Rg1and ginsenoside Rb1.The flow rate was 1.0 mL/min at 25 ℃.The detection wavelength was set at 203 nm.The HPLC was carried out on kromasil TMC18 column with acetonitrile-0.085% phosphonic acid(17∶ 83)as mobile phase for ferulic acid,the detection wavelength was at 316 nm,the flow rate was 1.0 mL/min and the column temperature was maintained at 35 ℃.RESULTS The linearity of notoginsenoside R1、ginsenoside Rg1、and ginsenoside Rb1 was in the ranges of 0.922-5.534 μg,1.337-8.021 μg and 1.032-6.182 μg,respectively.Their average recoveries were 99.47%,99.02%and 99.12%,respectively.The linearity of ferulic acid was in the range of 50.72-304.32 μg.The average recovery was 99.32%.CONCLUSION The assay demonstrates that the method is simple,has adequate accuracy and selectivity to quantify the four active components in Huoxue Zhitong Capsules.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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