Title: Apoptosis of type II alveolar epithelial cells in rat model of chronic obstructive pulmonary disease and the effects of inhaled glucocorticoid
Abstract: Objective To investigate the apoptosis of type Ⅱ alveolar epithelial cells(AECⅡ) in rat model of chronic obstructive pulmonary disease(COPD) and observe the effects of inhaled glucocorticoid on AECⅡ apoptosis.Methods A rat model of COPD was established by tobacco smoke inhalation and intratracheally administered lipopolysaccharide.Forty-eight rats were randomly divided into eight groups,ie.a sam control group,a budesonide(BUD)control group,a 15-days group of COPD,a 15-days group with low dose BUD,a 15-days group with high dose BUD,a 30-days group of COPD,a 30-days group with low dose BUD and a 30-days group with high dose BUD.The rats were sacrificed on 15th and 30th day respectively to obtain the pulmonary tissue.The degree of alveolar destruction was observed by HE stain and the apoptosis of AECⅡ was detected by immunohistochemistry and TUNEL methods.Results The degree of alveolar destruction and the apoptosis of AECⅡ were significantly increased in COPD rats in a time-dependent manner than those in the BUD and sam control groups(both P0.01) with a significantly positive correlation between each other(P0.01),Inhaled glucocorticoid could decrease the degree of alveolar destruction(P0.01) and the apoptosis of AECⅡ in COPD rats significantly(P0.01)in a dose-dependent manner.Conclusion The apoptosis of AECⅡ may be involved in the pathological process of COPD through alveolar destruction,and inhaled glucocorticoid can prevent alveolar destruction and the apoptosis of AECⅡ in COPD rats,which provide futher evidence for inhaled glucocorticoid in COPD treatment.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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