Title: Determination of Scutellarin and Chlorogenic acid in Qingkailing Granules by HPLC
Abstract: Objective To establish determination of scutellarin and chlorogenic acid in Qingkailing Extract by HPLC.Methods Kromasil C18 column and the mobile phase with methnol-water-phosphoric acid(20∶80∶0.2)with the flow rate 1.0 ml/min and column temperature 25℃ and the detection wavelength at 327 nm were applied for the determination.Results The linear range of scutellarin in the range of 0.090~0.540μg,r=0.9999,and its average recovery rate 100.3%(RSD=0.99%);while the linear of chlorogenic acid in the range of 0.196~1.176μg,r=0.9999,and its average recovery rate 99.9%(RSD=1.06%).Conclusion The method is sensitive,simple,accurate,so HPLC can be used for the determination of preparations and the quality control in Qingkailing Extract.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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