Title: Effect of Xinnaoshutong Capsules on oxidation injury and apoptosis after cerebral ischemia and reperfusion in rats
Abstract: Objective To study the protective effect and the anti-oxidation mechanism of Xinnaoshutong Capsules on neurons in rats with focal cerebral ischemia and reperfusion. Methods Rat model of focal cerebral ischemia and reperfusion was established by thread ligation in middle cerebral artery. After 24 hours, the morphological changes of rat cerebral cortical neurons (HE staining) were observed under optical microscope, apoptosis of cortical neurons was detected by TUNEL, the content of MDA、SOD、LD and LDH in ischemic cerebral brain were detected by spectrophotometry. Results Compared with the control group, there were obvious neurological deficits in the ischemia and reperfusion model groups. The significant ischemic morphologic changes in cerebral cortex were observed, such as neuronal edema and amount of apoptosis neurons. The content of MDA was much higher, whereas that of SOD was much lower in ischemia cerebra (P<0.01). The neurological deficits of middle cerebral artery occlusion (MCAO) rats could be improved and the neuronal edema and neuronal apoptosis of cortex could be lessened in the Xinnaoshutong Capsules group. Compared with the control group, MDA、LD and LDH were decreaced, and SOD was increased significantly in the Xinnaoshutong Capsules group (P<0.05~0.01). Conclusion Xinnaoshutong Capsules have the effect of lightening the injury degree of brain tissues and decreasing neuronal apoptosis after cerebral ischemia and reperfusion. The mechanism might be related to regulating oxidative stress response and elevating the free radical scavenging capability to alleviate cerebral injury.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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