Title: PROTECTION OF GINSENOSIDE Rg1 AGAINST-AMYLOID PEPTIDE-INDUCED NEUROTOXICITY IN MICE HIPPOCAMPAL NEURONS
Abstract: Objective To investigate the protection of ginsenoside Rg1 against-amyloid peptide β25-35(Aβ25-35)-induced neurotoxicity in mouse hippocampal neurons and the blocking effect of ICI182,780.Methods C57BL/6 female OVX mice were infused with Aβ25-35 into lateral cerebral ventricle to establish a model of Alzheimer disease.Rg1 was administrated intraperitoneally in the absence or presence of ER antagonist ICI182,780.Morris water maze were used to determine spatial learning and memoryability.RT-PCR was used to observe the changes of bcl-2 gene expression in the hippocampus.Results Aβ25-35 led to the increase of mice latency and total distance(F=11.34,10.90;q=6.184,5.905;P0.05) and decrease of the number of times crossing platform(F=10.90;q=6.023;P0.05).Moreover,it reduced the gene expression of bcl-2(F=18.47,q=6.082,P0.05).Compared with Aβ25-35 group,ginsenoside Rg1 significantly ameliorated the increase of latency and total distance(q=5.478,6.097;P0.05) and the reduction of platform crossing times(q=6.023,P0.05),reversed Aβ25-35-induced down-expression of bcl-2 in the hippocampus neurons(q=9.661,P0.05).The effects of Rg1 were blocked by the ER antagonist ICI182,780(q=5.360-7.482,P0.05).Conclusion Ginsenoside Rg1 may attenuate Aβ25-35-induced neurotoxicity in the hippocampal neurons,which might be related to the activation of ER signalling pathway.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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