Title: The protective effect of tripchlorolide on the hippocampal neurons of the Alzheimer′s disease rat model
Abstract: Objective To explore the protective effect of tripchlorolide(T4) on the hippocampal neurons of the Alzheimer's disease(AD) rat model and its possible mechanism. Methods Dorsal hippocampus microinjection of 10 μg aggregated beta-amyloid(Aβ)25-35 was performed by stereotaxic technique to establish the rat model of AD. Rats were divided into 5 groups: normal group, sham operation group, model group, T4 low dose(5 μg/kg)group, T4 high dose(20 μg/kg)group. The expression of nuclear factor(NF)-κB was detected by Western blot. The levels of tumor necrosis factor(TNF)-α and interleukin(IL)-1β were measured by enzyme-linked immunosorbent assay, and the apoptosis of hippocampal neurons was evaluated by Tunel staining. Results The expression of NF-κB,the TNF-α and IL-1β level in T4 group were significantly lower than those in model group. The apoptosis of hippocampal neurons in T4 groups decreased significantly compared with model group(P0.05). The T4 high dose group was especially significant(P0.01). Conclusions T4 had obvious protective effect on hippocampal neurons of AD model rat, and the mechanism may be due to decreasing the levels of TNF-α and IL-1β by inhibiting NF-κB activation.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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