Title: cDNA cloning and biological information analysis of cattle RXRG gene
Abstract: 【Objective】 Tow cDNA sequences of the RXRG gene were obtained and analyzed in biological information.【Method】 The total RNA was extracted from cattle muscle tissue.This experiment cloned cattle RXRG gene cDNA by RT-PCR,and the products were cloned in PMD19-T vector and sequenced.These two sequences were connected by DNAMAN software,and the connected sequence was analyzed by the DNAMAN software and online tool in biological information.The mRNA content was tested in kidney,liver,testicle,lung,spleen,rumen,uterus,small intestine,heart,and ovary and muscle tissues of cattle.【Result】 A 1 811 bp long cDNA sequence was obtained.The analysis of amino acid sequence indicated that cattle RXRG gene consisted of 10 exons and coded 463 amino acids.Homologous comparison with some animals indicated that cattle RXRG cDNA shared 89.5%,93.4%,87.2%,83.9%,85.2% similarity in nucleic acid sequence with human,pig,macaca,mouse and rattus.【Conclusion】 A 1 811 bp long cDNA sequence of RXRG gene was cloned.RXRG cDNA was found in muscle tissue by the analysis of its expression in various tissues,but not found in other tissues.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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