Title: Direct sequencing for katG and inhA genes of multi drug resistant isolates of mycobacterium tuberculosis
Abstract:Objective To characterize the katG and inhA genetic variations in multi drug resistant isolates of M. tuberculosis. Methods The amplicons were cloned and sequenced by ABI 377 automatic sequencing syst...Objective To characterize the katG and inhA genetic variations in multi drug resistant isolates of M. tuberculosis. Methods The amplicons were cloned and sequenced by ABI 377 automatic sequencing system. Results All isolates of M. tuberculosis with MIC for INH ≥1 μg/ml had point mutations and insertion in katG gene. Fourteen of 15 isolates (86.7%) had 463 codon (Arg→Leu) and 315 (Ser→Asn/Thr) mutations. Nine of 13 mutation positions were not reported previously. All 18 isolates with MIC≥1 μg/ml had one or more variations in inhA. The variations consisted of one or more base pairs deletions or point mutation. Each strain had its unique mutation. Seven of 10 isolates had both katG and inhA mutations. Conclusion Genetic variants of M. tuberculosis are significantly different from those of foreign strains.Read More
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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Cited By Count: 1
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