Title: Effects of bFGF gene transfection on the migration of vascular endothelial cells and its mechanism
Abstract: Objective To investigate the effects and its posssible mechanisms of basic fibroblast growth factor(bFGF)gene transfection on the migratory activity of vascular endothelial cells(HUVECs).Methods Eukaryotic expression plasmid pcDNA3.1-bFGF was constructed by gene subclone,and then was transfected into HUVECs by liposome mediating,RT-PCR and ELISA were used to determine the expression of bFGF mRNA.Transwell Chamber assay was performed to detect the cell migratory capacity changes of HUVECs after bFGF gene transferred.The expression level of Raf-1,extracellular regulated protein kinases 2(ERK2) and focal adhesion kinase(FAK) protein were assessed by Western blot.Results pcDNA3.1-bFGF was successfully constructed,bFGF mRNA and protein both increased significantly after the transfection.bFGF gene transfection promoted the migratory capacity of HUVECs in vitro,and up-regulated the expression of ERK2 and FAK protein ERK2 and FAK protein,but not affected the expression of Raf-1 protein.Conclusions The transfection of bFGF gene can promote the migration of vascular endothelial cell,its mechanisms might be associated with up-regulation of the expression of ERK2 and FAK protein.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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