Title: Inhabitory effects of basic fibroblast growth factor small interfering RNA on expression of vascular endothelial growth factor in pancreatic carcinoma cells
Abstract:Objective To investigate the effects of basic fibroblast growth factor (bFGF) small interfering RNA (siRNA) on the expression of vascular endothelial growth factor (VEGF) in pancreatic carcinoma cells...Objective To investigate the effects of basic fibroblast growth factor (bFGF) small interfering RNA (siRNA) on the expression of vascular endothelial growth factor (VEGF) in pancreatic carcinoma cells.Methods The expression and secretion of VEGF and bFGF in pancreatic carcinoma cells were inhabited by VEGF siRNA and bFGF siRNA.The expression of VEGF in pancreatic carcinoma cells was detected by using reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA).Results Both VEGF siRNA and bFGF siRNA significantly inhabited the expression of VEGF mRNA in Panc-1 and PCT-3 cells.Both VEGF siRNA and bFGF siRNA significantly inhabited the expression of VEGF in sw1990 and PCT-3 cells.Conclusion The bFGFsiRNA inhabited the expression of VEGF mRNA and VEGF in pancreatic carcinoma cells.
Key words:
Pancreatic carcinoma; Vascular endothelial growth factor; Basic fibroblast growth factorRead More
Publication Year: 2012
Publication Date: 2012-11-08
Language: en
Type: article
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