Title: Mechanism of silybinin on hepatic fibrosis in rats
Abstract: Objective To explore the effects of silybinin on hepatic fibrosis in rats. Methods Seventy female Wistar rats were randomly divided into normal control group(n=5),hepatic fibrosis group(n=33)and silybinin group(n=32).Carbon tetrachloride was injected for establishing hepatic fibrosis model in hepatic fibrosis group,while the silybinin 50 mg/(kg·d)was orally administrated simultaneously based on the injection of carbon tetrachloride in silybinin group.At the end of week 1,2,4,6,rats were killed under pyrogen-free conditions for collecting blood and liver tissues,respectively.The pathological and histological changes were observed.The intensity of the expression of connective tissue growth factor(CTGF)protein in liver tissues was assessed.The blood endotoxine(ET)and hydroxyproline(Hyp)and maleic dialdehyde(MDA)and alanine aminotransferase(ALT)were measured. Results Compared with normal control group,all indexes significantly increased in hepatic fibrosis group and silybinin group,and ET increased ahead of CTGF.Compared with hepatic fibrosis group,pathological injury of liver ameliorated in silybinin group,endotoxin and ALT levels in plasma,MDA and Hyp in liver tissues decreased in different degree,and the expression of CTGF in liver tissue decreased significantly. Conclusion Silybinin can attenuate hepatic fibrosis by decreasing the plasma level of endotoxine and the expression of CTGF in liver tissues.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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