Title: Regeneration system development for different melon varieties
Abstract: The influence of plant growth regulator combination,Ag NO3 concentration and genotype of melon on shoot organogenesis were investigated. The optimal concentrations of growth regulators were MS + 1.0 mg·L-16-BA + 0.5 mg·L-1ABA.The adventitious buds regeneration rates of‘IVF501',‘IVF509',‘IVF525',and‘IVF604'were 88.00%,79.60%,76.50%,and 74.75%,respectively. Different concentrations of Ag NO3 influenced melon adventitious bud regeneration. 1.0mg·L-1Ag NO3 effectively inhibited the callus formation and reduced the occurrence of the vitrification,the concentration of2.0 mg·L-1inhibited the callus formation,but at the same time reduced the rate of adventitious bud regeneration. The shoot elongation medium was MS + 0.05 mg·L- 16-BA. The rooting medium was MS + 0. 4 mg·L- 1IAA. It took about 50 to 60 days to obtain plantlet for‘IVF501'and 60 to 75 days for‘IVF525'from explants.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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