Title: Simultaneous determination of contents of protocatechuic acid,syringin and chlorogenic acid in Tianma toufengling capsules by HPLC
Abstract: Objective To develop a HPLC method for the determination of protocatechuic acid,syringin and chlorogenic acid in Tianma toufengling(traditional Chinese medicines) capsules.Methods The chromatographic separation was achieved on a Diamonsil C18(200 mm×4.6 mm,5 μm)column with a mobile phase of methanol-acetonitrile-0.05% phosphoric acid solution(V∶V∶V=20∶5∶174).The flow rate was 1.0 mL·min-1 and the column temperature was 35 ℃.Results The calibration curve was linear within the range of 2.40-24.0 mg·L-1(r=0.999 6)for protocatechuic acid,5.20-52.0 mg·L-1(r=0.999 7)for syringin and 26.6-266 mg·L-1(r=0.999 5)for chlorogenic acid,respectively.The recoveries of protocatechuic acid,syringin and chlorogenic acid were 101.0%(RSD=0.86%,n=9),100.3%(RSD=1.9%,n=9)and 101.5%(RSD=1.4%,n=9),respectively.Conclusions The method is accurate,sensitive and specific.It can be used for the quality control of Tianma oufengling capsules.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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