Title: Effect of hepatocytes transplantation through abdominal injection in rats with acute liver failure
Abstract: Objective To observe the effect of hepatocyte transplantation(HCT) through abdominal injection in rats with acute liver failure(ALF).Methods Rat hepatocytes were isolated with collagenase digestion.ALF rats were induced by D-GalN.HCTs were performed 24 h after the models were established.Rats were distributed in HCT group(Group A,n=20),model control group(Group B,n=20),angroup(group C,n=10) at random.Group A received 2.5×107 hepatocytes for every rat by abdominal injection.The 48 h liver function after HCT was assessed in group A,B and C.The 7 day survival rate after HCT was observed in group A dnormal control and B.Liver histology was observed for all rats.Result The survival rates of isolated hepatocytes was 87%~95%.About 7.8×107 hepatocytes could be isolated in one rat.Group A and B showed a higher serum GPT,GOT and AMM significantly than that of group E(P0.01);but there was no difference between group A and B(P0.05).The 7 day survival rate of group C was much higher than that of group D(50.00% vs 14.28%,P0.01).The liver histology was improved in group A,compared with group B 7 d after HCT.Conclusion HCT through abdominal injection is effective for ALF induced by D-GalN in rats.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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