Title: Severe Burn Induces Endoplasmic Reticulum Stress in Rat Cardiomyocytes
Abstract:Objective To study the effect of severe burns on induction of endoplasmic reticulum stress in rat cardiomyocytes.Methods Healthy adult Wistar rats were randomly divided into the control group(without ...Objective To study the effect of severe burns on induction of endoplasmic reticulum stress in rat cardiomyocytes.Methods Healthy adult Wistar rats were randomly divided into the control group(without burns),the burn group(subjected to a 30% total body surface area(TBSA)Ⅲ degree scalding) and the treatment group(treated with intraperitoneal injection of taurine(200 mg/kg) immediately after scald injury).The myocardia tissue samples were harvested at 1,3,6, 12 and 24 postburn hours(PBH).The cardiac troponin T(cTnT) and tumor necrosis factor alpha(TNFα) contents of blood plasma were measured by enzyme linked immunosorbent assay method(ELISA) and radioimmunoassay(RIA),respectively,RTPCR and immunohistochemistry were applied to monitor the gene expression changes of glucose-regulated protein 94(GRP94).Results The content of cTnT of blood plasma rose significantly within 3 postburn hours(P0.01),the gene expression of GRP94 and caspase-3 activity in 3 postburn hours rose and reached a peak after 12 h,while the GRP94 gene expression fell down dramatically in the group receiving taurine treatment.Conclusion Severe burn can induced endoplasmic reticulum stress in rat cardiomyocytes.Taurine has notable protective effect on the myocardial injury during the early postburn stage.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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