Title: Cloning the HSP20 Gene of B.bigemina Isolated in Xinjiang and Construction of the Eukaryotic Expression Plasmid
Abstract: The special primers were designed by the heat shock protein gene sequence of B.bigemina pubulished in GenBank.and a 699 bp HSP20 gene fragment was amplified by PCR from the whole blood genome in the infected cattle.The PCR product was cloned into a pMD18-T vector,then was sequenced and identified.The 699bp HSP20 gene was then cloned into Eukaryotic vector pcDNA3.1(+)to construct a eukaryotic expression plasmid pcDNA3.1-HSP20 before sequencing and identifying again.Following the pcDNA3.1-HSP20 was transiently expressed in mice injected by invena caudalis,the total RNA was abstructed from murine liver and a 534 bp of bright stripe was found by RT-PCR amplification,suggesting the eukaryotic expression plasmid was successfully constructed.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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