Title: Simultaneous determination of the contents of chlorogenic acid and caffeic acid in Solanum lyratum Thunb. by RP-HPLC
Abstract:Objective To establish an HPLC method for simultaneous determination of the contents of chlorogenic acid and caffeic acid in Solanum lyratum Thunb.Methods The operation was carried out on Diamonsil-C1...Objective To establish an HPLC method for simultaneous determination of the contents of chlorogenic acid and caffeic acid in Solanum lyratum Thunb.Methods The operation was carried out on Diamonsil-C18 column(250 mm×4.6 mm,5 μm)with the mobile phase consisting of a mixture of methanol-0.05% phosphoric acid(V∶ V=25∶ 75).The flow rate was 1.0 mL · min-1,UV detection wavelength was set at 327 nm and the column temperature was 45 ℃.Results The calibration curve of chlorogenic acid was linear in the range of 0.5-32.0 mg · L-1(r=0.999 9).The mean recovery of chlorogenic acid was 99.9%,RSD was 1.52%.The calibration curve of caffeic acid was linear in the range of 10.0-640.0 μg · L-1(r=0.999 9).The mean recovery of caffeic acid was 99.9%and RSD was 1.35%.Conclusions The HPLC method is found to be simple,accurate,sensitive and reproducible.It can be used for the determination of the contents of chlorogenic acid and caffeic acid in Solanum lyratum Thunb.Read More
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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