Title: Effects of genistein on induced apoptosis of cultured human RPE cells
Abstract: Objective To investigate the mechanism and effects of genistein(Gen)on induced apoptosis of cultured human retinal pigment epithelium(RPE)cells.Methods The 0 μmol·L-1,25 μmol·L-1,50 μmol·L-1,100 μmol·L-1 and 200 μmol·L-1 Gen were used to treat RPE for 24 hours,respectively.MTT was used to detect cell activity,flow cytometry to detect cell cycle for quantitatively analyzing apoptosis,and immunoblotting to detect expression and activity of extracellular regulated protein kinases(ERK)and p38MARK.Results After different concentrations of Gen treating RPE for 24 hours,cell growth was obviously inhibited,inhibitive rates were 6.44%,14.71%,28.97% and 45.75%;Rates of cell apoptosis were obviously increased,and were 16.87%,19.44%,23.14% and 34.81%.Cell cycles of S stage and G2/M stage were blocked;Cells at G0/G1 stage were decreased.Expression of p-ERK1/2 and p-p38 were with down-regulation.There was negative correlation between activation of ERK1/2 and cell apoptosis induced by Gen,and activation of p38 may also have relation with apoptosis.Conclusion Gen can inhibit proliferation of RPE cells,induce cell apoptosis and there is dose-depend manner,which mainly have biological effect by ERK pathway.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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