Title: Study of mechanism of action of specific inhibitor of COX-2 on Egr-1
Abstract: Objective:To study the effects of specific inhibitor of COX-2 celecoxib in different concentration on cell apoptosis,cell cycle and expression of Egr-1 of A549 cells,and to investigate the molecular mechanism of anti-tumor effects.Methods:A549 cells were cultured in vitro and treated by 25.0,50.0 μmol/L and 100.0 μmol/L celecoxib.Then the TUNEL assay was used to detect apoptosis;FCM assay was used to determine cell cycle,and RT-PCR assay was performed to evaluate the Egr-1 and Fas mRNA expression levels.Results:Treated by celecoxib in the concentration of 25.0,50.0 μmol/L and 100.0 μmol/L,the cell cycle was arrested in c phase in A549 cells respectively;the apoptotic index of A549 cells were 10.67%±1.46%,18.83%±2.54% and 25.67%±2.10% respectively,and the expression levels of Egr-1 and Fas mRNA were up-regulated synchronously.Conclusion:Celecoxib,a specific inhibitor of COX-2,can inhibit the proliferation of A549 cells by regulating the cell cycle and inducing the cell apoptosis.The molecular mechanism may be that COX-2 inhibitor up-regulates the expression of Fas by up-regulating the expression of Egr-1,and then induces the cell apoptosis by blocking the A549 cells in G0/G1 period.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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