Title: Effect of recombinant murine Interleukin 12(rmIL-12) on radiation protection and bone marrow hematopoietic function
Abstract: Objective To study the effect of recombinant mouse interleukin-12(rmIL-12) on survival and bone marrow hematopoietic function of gamma-ray irradiated mice.Methods Mice were randomly divided into three groups according to weight: irradiation control group,positive control group and rmIL-12 treatment group(200 ng/mouse).Sixty mice,twenty in each group(male:female=1∶1),were used to study the survival rate after total body irradiation(TBI) in 8.0 Gy while thirty female mice,ten in each group,were used to study the effect on hematopoietic function after TBI in 4.0 Gy.All mice were administrated 24 h and 1 h before the delivery of irradiation time and after irradiation of medication,once a day,for 7 days,via subcutaneous injection.The protection effect on survival in mice bone marrow mesenchymal stem cells(MSCs) cultured in vitro was observed in different irradiation doses.Results In 60Co gamma-ray radiation dose for 8.0 Gy,rmIL-12 obviously increased the survival rate,lifted and right-shifted its survival curve(Kaplan-meier curve)after TBI in mice.In 60Co gamma-ray radiation dose for 4.0 Gy,the number of white blood cells,platelets and reticulocytes in peripheral blood was increased by rmIL-12,and the content of DNA in bone marrow was obviously higher than in control group.Meanwhile,the final concentration of rmIL-12 ranged from 0.125 ng/ml to 32 ng/ml.Protection effect on the MSCs survival in different doses was observed in vitro.Conclusion rmIL-12 can increase the survival rate and accelerate the recovery of hematopietic function after total body irradiation(TBI) in mice,and also has the protective effect in the survival of vitro cultured MSCs.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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