Title: Study on Pharmacokinetics of Picroside I in Rats and Dogs
Abstract: Aim To establish a RP-HPLC method for determining plasma picroside-I concentration,and study the pharmacokinetics characters of picroside-I in rats and Beagle dogs.Methods Acetonitrile-water mixture was used as mobile phase,Agilent XDB C 18 column was selected.The wavelength of UV detection was 277nm.After one dose of Picroside-I were administered through injection to rats and dogs,RP-HPLC was used to determine Picroside-I concertration in plasma at different times.3p97 program was used to process concertation-time data.Results The lowest detectable concertration of Picroside I was 0.1μg·ml -1 .The recovery of the methode were more than 90%,RSD of intra-day and inter-day were less than 5% respectively.After one dose of Picroside-I injection,The concertration-time curves were both confirmed to two-compartment open models in rats and dogs.The main pharmacokinetic parameters such as T 1/2β ,CLs and MRT showed no significant difference between rat and dog.Conclusion The method is accurate,sensitive and suitable for the measurement of plasma Picroside I concentration.The pharmacokinetics characteristics showed Picroside I was metabolized rapidly in rat and dog in vivo,distributed abroad,and eliminated slowly.There was no difference in the pharmacokinetic between rats and dogs.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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