Title: Effect of p53 Inhibitor-α on Adriamycin Induced Apoptosis of Cultured Human Skin Keratinocytes in Vitro
Abstract:Objective To investigate the effect of p53 inhibitor-α(PFT-α)on adriamycin induced apoptosis of cultured human skin keratinocytes in vitro.Methods Human skin keratinocytes were cultured with 0,4,8,12m...Objective To investigate the effect of p53 inhibitor-α(PFT-α)on adriamycin induced apoptosis of cultured human skin keratinocytes in vitro.Methods Human skin keratinocytes were cultured with 0,4,8,12mg/L PFT-α and adriamycin.Cell proliferation was detected by colorimetric assay of MTT.The optical density(OD) values were read on a scanning multiwell spectrophotometer(ELISA reader).Apoptosis rate of human skin keratinocytes was detected by flow cytometry.Results The proliferation of human skin keratinocytes was inhibited by adriamycin.If cells were pre-incubated with 8,12mg/L PFT-α,OD value was significant higher and apoptosis induced by adriamycin was suppressed compared with control group(P0.05).Conclusion This study suggested that PFT-α could partially inhibit adriamycin induced apoptosis of cultured human skin keratinocytes.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot