Abstract: The research aimed to investigate the optimum conditions for extracting catechin in tea by solvent extraction method.[Method]With ethanol as leaching liquor,the method of vanillin-hydrochloric acid was used to determine the content of catechin in tea and the optimum technique conditions for extracting catechin in tea was confirmed by orthogonal test.[Result]The maximum absorption wavelength of catechin solution was 505 nm.The regression equation between catechin concentration and absorbance was:A=0.017 49X+0.044 73(R2=0.999 7).The results of orthogonal test showed that the optimum extracting and purifying conditions for catechin were as follows: extracting with 80% ethyl alcohol as solvent for 2 times,extracting at the temperature of 60 ℃ for 35 min,the ratio of material to liquid of 1∶13.The factors that affected the content of catechin were as follows: ethanol concentrationextraction time extraction temperature the ratio of material to liquid.[Condusion]The research laid the foundation for the application of catechin.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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