Title: Study on the New Extraction Process of Catechin
Abstract: To study the optimum conditions for extracting catechin in tea by solvent extraction method.With ethanol as leaching liquor.The method of vanillin-hydrochloric acid was used to determine the content of catechin in tea and the optimum technique conditions for extracting catechin in tea was confirmed by orthogonal test.The maximum absorption wavelength of catechin solution Was 506 nm.The regression equation between catechin concentration and absorbance was: A=0.058X+0.059,R2=0.994.The results of orthogonal test sho-wed that the optimum extracting and purifying conditions for catechin were as follows: extracting with 60 % ethyl alcohol as solvent at the pH of 4,extracting at the temperature of 60 ℃ for 30 min,the ratio of material to liquid of 1∶15(g/mL).The factors that affected the content of catechin were as follows: pH valueethanol concentrationextraction timethe ratio of material to liquidextraction temperature.By studying the extraction and determination of catechin in tea.We determined a economic,efficient and practical extraction and separation process.And established a simple,accurate of catechin determination methods.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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