Title: Sodium nitroprusside inhibits the expression of intercellular adhesion molecule-1 induced by lipopolysaccharide in primary cultured rat hepatocytes
Abstract: AIM To investigate the relation between nitric oxide (NO) and intercellular adhesion molecule 1 (ICAM 1) expression. METHODS The primary cultured rat hepatocytes were incuba ted with sodium nitroprusside (SNP), and the 50% lethal concentration (LC 50 ) was measured by microculture tetrazolium assay (MTT). The expression of ICAM 1 in hepatocytes was detected by immunocytochemistry when the cells were incubated with SNP in 0, 100, 200, and 300 μmol·L -1 alone, or lipopolysaccharide (LPS) 10 mg·L -1 +SNP in fresh media for 4, 8 or 24 h, respectively. RESULTS Exposure of hepatocytes to SNP for 24 h, the value of LC 50 and 95% confidence limits was 597 (532-670) μmol·L -1 . ICAM 1 positive hepatocytes in negative control were (3.1± 1.8)%, and in SNP groups were (4.5± 2.2)%. ICAM 1 expression could be up regulated by LPS in hepatocytes positive cells (98.7± 2.6)% . SNP(100, 200, 300 μmol·L -1 ) could down regulated ICAM 1 overexpression stimulated by LPS to (68.6±3.4)%, (42.4±5.6)%, (20.1±1.8)% for 24 h incubation. CONCLUSION NO donor SNP can suppress ICAM 1 overexpression in primary cultured rat hepatocytes stimulated by LPS.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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