Title: Effects of memantine on expression of Caspase-3 and MDA in rats after ischemia-reperfusion
Abstract: Aim: To investigate the inhibition of NMDA receptor antagonist memantine on the cerebral ischemic injury of delay in rats.Methods:One hundred and thirty-five male Wistar rats were randomly divided into 3 groups:sham-operation group,model group,and intervention group.The models of cerebral ischemia-reperfusion injury rats were established by the middle cerebral artery occlusion(MCAO).The rats of intervention group were treated with memantine.The changes of cell morphology and the expression of Caspase-3 in cerebral cortex neurons were observed by HE staining and immunohistochemistry technique,respectively.The contents of Caspase-3 and MDA were detected by spectrophotometer.Results:The expression of Caspase-3,Caspase-3 enzyme activity and the level of MDA among the 3 groups at 12,24,and 48 h were different(F=63.900,246.589,437.277;41.151,82.116,106.407;387.615,209.846,475.285,P0.001).The expression levels of Caspase-3 and MDA in intervention group were lower than those of model group but higher than those of sham-operation group(P0.05).Conclusion:NMDA receptor antagonist could suppress the expression of Caspase-3 and MDA in ischemia-reperfusion brain and inhibit injury of delay in rats to play a neuroprotective role.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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