Title: Effect of Heme Oxygenase-1 Gene on Cognitive Function and Oxidative Stress During Chronic Cerebral Ischemia in Rats
Abstract: Objective:To investigate the effect of heme oxygenase-1(HO-1) gene transfected by a recombinant adeno-associated virus vector injected in rats hippocampus on cognitive function and oxidative stress during chronic cerebral ischemia(CCI).Methods:Thirty male Wistar rats were divided into sham operation group(SH),two-month CCI group(2-VO) and two-month CCI +HO-1 group(2-VOH).The HO-1 gene was injected into hippocampus in 2-VOH group.Seven days later,CCI was induced by permanent occlusion of bilateral common carotid arteries in rats.Learning and memory ability was evaluated by Morris water maze(MWM).After 8 weeks,the level of HO-1 protein in hippocampus was determined with Western blotting.SOD and MDA Test Kit was used to test levels of MDA,NO and activities of SOD in hippocampus.Results:The ability of rats at two months after ligation of bilateral common carotid arteries was significantly lower in 2-VO group than that in SH group(P0.01);In the 2-VOH group,the escape latency was significantly shorter than that in 2-VO group(P0.01);The expression of HO-1 protein was very low in SH and 2-VO group,but it was significantly increased in 2-VOH group(P0.01);SOD activity was up-regulated and MDA,NO levels were downregulated in the hippocampus in 2-VOH group compared to the 2-VO rats(P0.05).Conclusion:These results indicate that HO-1 gene transfected by a recombinant adeno-associated virus vector can improve rats cognitive function and reduce oxidative stress during chronic cerebral ischemia.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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