Title: Reduction of liver fibrosis in rats after transplantation of mononuclear bone marrow cell through portal vein
Abstract: Objective To study contrastingly the curative effect of mononuclear bone marrow cell transplantation via portal vein in the rat models with liver cirrhosis.Methods Rat hepatic cirrhosis models were induced by carbon tetrachlorid(e CCl4).After 8 weeks,twenty rat models were pathologically confirmed as hepatic cirrhosis,which were randomly divided into treatment group and control group(each with 10 cases).Mononuclear bone marrow cell were infused into the rats in the treatment group via the portal vein,while normal saline was infused instead in control group.CCl4were hypodermically injected in these rats,twice a week and fed with original conditions for 4 weeks.When the trial ended,all the rats were killed;liver samples were stained with HE or Masson trichrome,respectively.The evaluation indexes include: ①The general conditions of the rats;②The liver fibrosis index(hyp and collagen fibre);③The liver pathological grades index.Results ① The general conditions of the rats in treatment group improved markedly,but no in control group.②Hyp was(0.50±0.12) g in treatment group,and(0.59± 0.34) g in control group;While percentage of collagen fibre was(3.75 ± 0.98)% and(5.02 ± 0.44)%,respectively.There was significant difference between two group(s P 0.05).③Liver pathological grades were improved significantly from Ⅳ grade to Ⅲ grade partly in treatment group(P 0.05);while no obvious improvement in control group(P 0.05).There was significant difference between treatment group and control group(P 0.05).Conclusion Transplantation of intrahepatic mononuclear bone marrow cell can reduce liver fibrosis in mice.The research is developing a new method for clinical liver cirrhosis.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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