Title: Determination of puerarin in Xingkeshu capsule by HPLC
Abstract: OBJECTIVE To determine the contents of the puerarin in Xingkeshu capsule by HPLC.METHODS RP-HPLC was used with a column of ODS and a mobile phase of methanol-water(25∶75),the flow rate was 1mL·min-1,the detection wavelength was 250nm and the column temperature was 25℃,the injection volume was 20μL.RESULTS The retention time of the puerarin was about 13.25min,the puerarin was within 0.2~3.2μg·mL-1.The correlation coefficient was 0.9999.The average recoveries of paeoniflorin were 99.98%(n=5,RSD=1.13%).CONCLUSION This method was accurate,reliable and can be used to control puerarin in Xingkeshu capsule.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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