Title: Determination of Puerarin in Puerarin Injection by HPLC
Abstract: Objective To establish the determination method of puerarin in puerarin injection.Methods HPLC was used on ODS C_(18) column,methanol-1%glacial acetic acid(1:3) was used as a mobile phase.The detection wave-length was at 250nm.Results The detection range was 0.32~1.60ug.There was a good linear relationship between consistence and apex-area.The regress equation was Y=2E+06X+33911,r=0.999 9.The recovery of the added sample was 99.82%,and RSD=0.98%.Conclusion This method was simple and reliable,and could be used for the control quality of puerarin injection.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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