Title: Effects of Liuwei Dihuang Wan on AQP1 expression of lung and kidney in kidney-yin deficiency model rats
Abstract: Objective: To observe the intervention effects of Liuwei Dihuang Wan(LW) on the expression of AQP1 in lung and kidney in kidney-yin deficiency rats.Methods: forty SD rats were randomly divided into: normal control group,thyroxine group(n=10) and thyroxine group(n=30); Model of kidney yin deficiency rats was produced by ig administrating tetraiodothyronine per day.Four weeks after this procedure,rats in thyroxine group were randomly classified into thyroxine group,and two treatment groups(high dose group and low dose group); treatment groups were ig administered once daily for 4 weeks.Observing records of each rats' body weight,water quantity and general symptoms and signs such as urine; After the last delivery,AQP1 mRNA was assayed by real time PCR.The protein was measured by Western Blot.Results: Compared with normal control group,the thyroxine rats had a low level of body weight,water intake and low level of urinary output(P0.01),AQP1 protein and mRNA(P0.01).Compared with the model rats,the protein levels of AQP1 and mRNA expression of AQP1 in lung and kidney were all significantly reduced after treatment with low or high dose of LW-medicated(P0.01).Conclusion: Liuwei Dihuang Wan can reduce AQP1 level in kindey-yin deficiency model rats.It is suggested that may be one of the fluid metabolism regulation mechanisms of Liuwei Dihuang Wan in kindey-yin deficiency.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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