Title: Cryopreservation of Scophthalmus maximus embryos by vitrification
Abstract: Vitrification solutions were examined for their suitability of cryopreservation of turbot (Scophthalmus maximus) embryos. PMP1 gave higher survival rate (75.68%) than other vitrification solutions and was considered to be suitable for the cryopreservation of turbot embryos. The freezing point of the vitrification solutions consisted of PG and MeOH in the proportion of 3:2 was measured when the vitrification solutions were cryopreserved. It showed that the vitrification solutions with a cryoprotectant concentration of over 41% have no freezing point and the freezing points of vitrification solutions containing 33.33% - 40% cryprotectants were between -32.4℃ and -65.4℃. The freezing point decreased with the increase of cryoprotectant concentration. The resistance of turbot embryos at different stages to the cryoprotectants was studied. It was found that the turbot embryos from 4-5 pairs somite to tail bud were more resistant to cryoprotectants and suitable to vitrify. A live embryo was obtained after cryopreservation in liquid nitrogen for 14h and was hatched out.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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Cited By Count: 7
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