Title: Gene expression profile of gastric tumorigenesis detected by cDNA microrray
Abstract: Purpose To establish the gene expression profiles of gastric carcinoma,and to identify gastric carcinoma-related genes.Methods The changes of gene expression profile between gastric carcinoma and adjacent normal tissue of gastric epithelia were analyzed by cDNA microarray which represents approximately 10 000 genes and 7 000 expressed sequence tags(ESTs) in this study. Reverse transcription polymerase chain reaction (RT-PCR) was employed to validate the relationship between gastric carcinoma-related genes and gastric carcinoma.Results 359 genes that were differentially expressed in cancer and non cancer tissues were identified; 271 genes were highly expressed2.0-fold in cancer tissues,88 genes were poorly expressed 2.0-fold in cancer tissues,respectively; 28 ESTs that were differentially expressed in cancer and non cancer tissues were identified; 24 ESTs were highly expressed 2.0-fold in cancer tissues,4 ESTs were poorly expressed 2.0-fold in non cancer tissues. Furthermore,we found carbonic anhydrase Ⅱ was poorly expressed in gastric carcinoma,and insulin-like growth factor-binding protein 4 (IGFBP4) was highly expressed in gastric carcinoma using RT-PCR.Conclusion These results suggest that carbonic anhydrase Ⅱ and IGFBP4 may be associated with carcinogenesis of gastric carcinoma,and those also provide useful clues for future cloning gastric carcinoma related genes.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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