Title: Gene expression analysis of pro-onc and tumor suppressor genes in gastric cancer by complementary DNA microarray
Abstract: Objective: To study the expressions of pro-onc and tumor suppressor genes in gastric cancer by cDNA expression array. Methods: The probes were prepared by labeling gastric cancer tissue mRNA and normal gastric mucosa mRNA with Cy5-dUTP and Cy3-dUTP separately through reverse transcription. The mixed probes were hybridized to the cDNA microarray, and the fluorescent signals were scanned to show the difference between 2 kinds of tissues. Results: The comparative analysis revealed that 13 out of 195 pro-onc and tumor suppressor genes had differential expression (2 fold or 0.5 fold), among which 6 were up-regulated (3.1%) and 7 down-regulated (3.6%). Conclusion: These studies indicate that the genes identified through this approach have potential value in the diagnosis and therapy of gastric cancer.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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