Title: 8-ohdG AND rOGG1mRNA EXPRESSION IN RAT WITH FOCAL CEREBRAL ISCHEMIA AND REPERFUSION
Abstract: Objectives: To invesitgate the change and effect of 8-ohdG and rOOG1 in rat with focal cerebral ischemia and reperfusion. Methods: Male Wistar rats weighting 250±30g were dividing into 2 groups; ①pure model group,② sham-operated group. The focal cerebral ischemia and reperfusion model was made by thread embolish of middle cerebral artery. Rats were killed at 6, 24 and 48 hours after ischemia/reperfusion. The brain was removed, sliced into 2-mm sections. The third 2-mm section was applied to DNA or RNA extraction. DNA was analyzed by HPLC with the electrochemical (EC) detection. Using a semi-quantitative reverse transcription-polymerase chain reaction assay on total mRNA from the ischemic tissue, OGG1 mRNA was determined in rat brains. Results: ①-The amount of 8-ohdG in pure model group after reperfusion was significantly increasing compared with sham-operated group (P0. 01). During reperfusion after 2 hours of ischemia with the passage of time, the amount of 8-ohdG in penumbra was reducing gradually. ②2During reperfusion after 2 hours of ischemia with the pasage of time, rOGGlmRNA expression in pure model group was increasing gradually, but there was a significant reduction compared with sham-operated group(P0. 01). Conclusions: Oxidative DNA damage and repair defect might play a vital role in regulating the neuron death during reperfusion after ischemia.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
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