Title: Comparison of Phage Amplified Biologically Assay with Routine Methods in Detection of Mycobacterium tuberculosis
Abstract: Objective To evaluate the clinical significance of Phage amplified biologically assay in rapid detection of Mycobacterium tuberculosis and to compare Phage amplified biologically assay with the other routine methods. Methods Totally 135 clinical specimens(sputum,pleural effusion,lavage fluid, and cerebrospinal fluid) from the patients with tuberculosis(pulmonary tuberculosis,tuberculous pleurisy and tuberculous meningitis) and 67 sputum specimens from patients without TB(21 cases of chronic bronchitis,20 cases of bronchiectasis,18 cases of lung cancer,and 8 cases of pneumonia) were simultaneously detected by the Phage amplified biologically assay,Bactec 960 culture method and smears test. Results The positive rates of 135 specimens detected by Phage amplified biologically assay,Bactec 960 culture method and smears test were 48.2%,44.4% and 28.2%,respectively.The detection rate of Phage amplified biologically assay was higher than that of smears test,and the difference was statistically significant.The positive coincidence rate was 58.5%(38/65),the negative coincidence rate was 95.7%(67/70),and the total coincidence rate was 77.8%(105/135).There were statistically significant differences between Phage amplified biologically assay and Bactec 960 culture method.The positive coincidence rate was 78.5%(51/65),the negative coincidence rate was 72.9%(51/70),and the total coincidence rate was 75.6%(102/135).Among the 75 samples with negative tuberculosis smears and Bactec 960 cultures,11 samples were positive tested by Phage amplified biologically assay,with the detection rate of 14.7%.The positive rates of 67 sputum specimens from patients without TB detected by the three methods were all 0%. Conclusions The results suggest that Phage amplified biologically assay is a sensitive and specific method in detection of Mycobacterium tuberculosis; moreover,it is an effective method in auxiliary diagnosis and differential diagnosis of tuberculosis.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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