Title: Study on Enzyme Process Extraction of Chlorogenic Acid from of Eucommia Ulmoides Leaves
Abstract: In this paper,the best operation condition of chlorogenic extraction from the eucommia ulmoides leaves was found through analyzing the influences of enzyme process: humidify with zero-water → cellulase(45 ℃) and pectolase(55 ℃) constant temperature destroying cell wall and hydrolysis of big molecular organics in 10 min →refluxing by zero-water(2 times,the time was 10 min each time)→vaccum evaporation→extraction by ethyl acetate(4 times,pH=3)→extraction with water(pH=7)→condensation→recrystallization(at purity 99.21%).This process method is simple and applicable.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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