Title: Establishment of callus induction and subculture system of Taxus chinensis var.mairei
Abstract: The callus induction and maintenance system of Taxus chinensis var.mairei was established in the experiment,the main results were as follows:(1) lateral buds were the best explants for the callus induction;(2) B_5 plus 2.0 mg·L~(-1) 2,4-D,1.0 mg·L~(-1) NAA and 1.0 mg·L~(-1) IAA was the best medium for callus induction;(3) B′_5 containing 4.0 mg·L~(-1) NAA,0.5 mg·L~(-1) 2,4-D,(0.2) mg·L~(-1) GA_3,500 mg·L~(-1) AC and 2000 mg·L~(-1) LH was the best medium for callus growth.The brownness of callus could be avoided or decreased to a certain degree when AC,LH and GA_3 were added to the mediums.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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