Title: The effects of amino guanidine on the expression of Caspase-1 and NF-κ b in diabetic rats’retina
Abstract: Objective To study the expression of caspase-1 and NF-κb in the retina of diabetic rats and to investigate the possible pathogenesis of diabetic retinopathy.Methods 60 wistar rats,8 were used as control. Diabetes were induced on the other rats by the intraperitoneal injection of streptozotocin (STZ) at a doze of 60 mgkg. Diabetes were characterized by an increase in blood glucose (16.65mmolL) 24~48 hours after injection. The successful diabetic rat models were randomly divided into diabetic mellitus (DM) group and amino guanidine (AG) group. DM and AG groups were subdivided into DM1,DM3,DM6 and AG1,AG3,AG6 groups according to the duration of diabetes. The expression of Caspase-1 and NF-κ b were studied by streptavidin-biotin complex (SABC) immunohistochemistry .And the effects of amino guanidine on the expression of Caspase-1 and NF-κ b were also studied. Result: no Caspase-1 positive cells were observed in the normal retina. In DM1 group, no obvious expression of Caspase-1 were found, while in DM3 group, positive expression of Caspase-1 were found in ganglion cell layer(GCL). And in DM6 group, positive expression extend to the inner nuclear layer(INL) and the outer nuclear layer(ONL).The positive expression of NF-κ b is low and only in INL and ONL of normal retina. It had no obvious change in DM1,and gradually increased in DM3 and DM6 .That is ,the expression mainly in INL, ONL and GCL in DM3, and extend to almost the whole retina in DM6 .Under the intervention of amino guanidine ,the expression of Caspase-1 and NF-κb were inhibited in each AG group. The distinction of positive expression between DM3 and AG3,DM6 and AG6 have statistical significance(P0.05) Conclusion: Diabetes elevated the expression of Caspase-1 and NF-κb, which may take part in the early events of the onset of diabetic retinopathy, the pathogenesis may be that Caspase-1 and NF-κb cause the apoptosis of retinal nerve cells. Amino guanidine may cure diabetic retinopathy through its inhibition on the expression of Caspase-1 and NF-κb.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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