Title: Relationship between methylenetetrahydrofolate reductase gene polymorphisms,levels of plasma homocystein and coronary heart disease
Abstract: AIM To investigate the relationship between methylenetetrahydrofolate reduetase(MTHFR) gene polymorphisms,plasma homocystein(HCY) and coronary heart disease(CHD).METHODS One hundred and seventy-eight subjects who had undergone coronary angiography in our hospital were included in this study.Cases(n=122) with ≥50% stenosis in at least one of the three coronary vessels were angiographically defined as having coronary heart disease(CHD),while subjects(n=56) without stenosis in all the three coronary vessels were included as controls.MTHFR gene C677T polymorphisms was determined with polymerase chain reaction(PCR) and restriction fragment length polymorphisms(RFLP).Plasma HCY levels were measured using high-performance liquid chromatography with fluorescence detection.RESULTS The mean plasma HCY was significantly higher in CHD patients than that in in controls(P0.05).The frequencies of MTHFR C677T homogenetic type and heterogenetic type in patients with CHD were 30% and 45% respectively,higher than those in control group(25% and 32%, respectively).The frequency of 677T allele was much higher than that in control group(P0.05) and plasma HCY levels were markedly higher in patients with TT genotype than those in patients with TC and CC genotype(P0.05).CONCLUSION MTHFR C677T gene polymrophism is closely associated with CHD.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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