Title: Correlation between plasma homocysteine level and mutation of 5, 10-methylenetetrahydrofolate reductase gene in middle and young people with ischemic cerebravascular diseases
Abstract: Objective To expore the correlation between plasma homocysteine (Hcy) level and mutation of 5,10-methylenetetrahydrofolate reductase (MTHFR) gene in middle-age and young patients with ischemic cerebravascular diseases (ICVDs).Methods Two hundred and twenty patients with ICVDs (≤ 55 years),admitted to our hospital from January 2010 to September 2012,were included as the case group while 203 subjects without stroke and coronary arterial disease matched with the case group for gender and age were collected as the control group.Plasma Hcy level was measured using fluorescence polarization immunoassay; and MTHFR c677T mutant was determined by polymerase chain reaction and restriction fragment length polymorphism.Results The mutated T allele frequencies in case and control groups were 59.8% and 45.8% with statistically significant difference (P<0.05); homozygous mutant TT and heterozygous mutant CT,homozygous wild type CC between the two groups were statistically different (P<0.05).The level of plasma Hcy were (32.16±19.52) μmol/L in the case group and (18.73±8.75) μmol/L in the control group with statistical significance difference (P<0.05).The level of plasma Hcy of case and control groups with TT type was significantly higher than that with CT and CC types (P<0.05).Multivariate Logistic regression analysis showed that age,hypertension,low-density lipoprotein cholesterol,diabetes mellitus,and high Hcy were risk factors for onset of ICVDs in middle-age and young patients.Conclusions High plasma Hcy level is a risk factor for onset of ICVDs in middle-age and young patients; MTHFR gene C677T homozygous mutation can induce an increase in plasma Hcy level,and there may be interactions with other factors.
Key words:
Ischemic cerebravascular disease; 5, 10-methylenetetrahydrofolate reductase; c677T mutant; Homocysteine; middle-aged and young people
Publication Year: 2014
Publication Date: 2014-05-15
Language: en
Type: article
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